HA-Akt DN (K179M)
(Plasmid #16243)

• Depositing Lab: Mien-Chie Hung
• Publication: Zhou et al J Biol Chem. 2000 Mar 17. 275(11):8027-31.

Backbone

• Vector backbone: pCMV5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Akt1
• Species: M. musculus (mouse)
• Mutation: Dominant negative (K179M)
• Entrez Gene: Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Philip Tsichlis
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry