pCR3.1 GSK3beta S9A HA
(Plasmid #15994)

• Depositing Lab: Morris Birnbaum
• Publication: Summers et al J Biol Chem. 1999 Jun 18. 274(25):17934-40.

Backbone

• Vector backbone: pCR3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GSK3beta
• Alt name: glycogen synthase kinase 3beta
• Species: H. sapiens (human)
• Insert Size (bp): 1500
• Mutation: S9A: constitutively active
• Entrez Gene: GSK3B
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Peter Klein, Univ of PA
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry