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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepMT2
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGSK3 alpha
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Alt nameGSK3a
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
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Entrez GeneGSK3A
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pMT2-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ORF is at 1189-2640.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GSK3 alpha pMT2 was a gift from Jim Woodgett (Addgene plasmid # 15896 ; http://n2t.net/addgene:15896 ; RRID:Addgene_15896) -
For your References section:
Molecular cloning and expression of glycogen synthase kinase-3/factor A. Woodgett JR. EMBO J. 1990 Aug . 9(8):2431-8. PubMed 2164470
