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PurposeExpression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 154013 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57 mini
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCS
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Alt namemulti-cloning site
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SpeciesSynthetic
- Promoter CAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
iOn-CAG∞MCS was a gift from Jean Livet (Addgene plasmid # 154013 ; http://n2t.net/addgene:154013 ; RRID:Addgene_154013) -
For your References section:
Direct Readout of Neural Stem Cell Transgenesis with an Integration-Coupled Gene Expression Switch. Kumamoto T, Maurinot F, Barry-Martinet R, Vaslin C, Vandormael-Pournin S, Le M, Lerat M, Niculescu D, Cohen-Tannoudji M, Rebsam A, Loulier K, Nedelec S, Tozer S, Livet J. Neuron. 2020 Aug 19;107(4):617-630.e6. doi: 10.1016/j.neuron.2020.05.038. Epub 2020 Jun 18. 10.1016/j.neuron.2020.05.038 PubMed 32559415
