-
PurposeVector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 154019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepUC57 mini
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namemRFP1
-
Alt nameRFP
-
SpeciesSynthetic
-
MutationAll TTAA in vector backbone were replaced by silent substitutions
- Promoter CAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCAGGS-5 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
LiOn*-CAG∞RFP was a gift from Jean Livet (Addgene plasmid # 154019 ; http://n2t.net/addgene:154019 ; RRID:Addgene_154019) -
For your References section:
Direct Readout of Neural Stem Cell Transgenesis with an Integration-Coupled Gene Expression Switch. Kumamoto T, Maurinot F, Barry-Martinet R, Vaslin C, Vandormael-Pournin S, Le M, Lerat M, Niculescu D, Cohen-Tannoudji M, Rebsam A, Loulier K, Nedelec S, Tozer S, Livet J. Neuron. 2020 Aug 19;107(4):617-630.e6. doi: 10.1016/j.neuron.2020.05.038. Epub 2020 Jun 18. 10.1016/j.neuron.2020.05.038 PubMed 32559415