Skip to main content
Addgene

AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR
(Plasmid #154003)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154003 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAAV
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CasRx
  • Alt name
    NLS-RfxCas13d-NLS
  • Species
    Synthetic
  • Insert Size (bp)
    3848
  • Promoter EFS

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

INSERT: CasRx driven by EFS promoter.
INSERT: DR-SgRNA_LacZ driven by U6 promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR was a gift from Hui Yang (Addgene plasmid # 154003 ; http://n2t.net/addgene:154003 ; RRID:Addgene_154003)

  • For your References section:

    Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver. He B, Peng W, Huang J, Zhang H, Zhou Y, Yang X, Liu J, Li Z, Xu C, Xue M, Yang H, Huang P. Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 10.1007/s13238-020-00700-2. 10.1007/s13238-020-00700-2 PubMed 32185621
Related items:
Commonly requested with: