WT N-His Ana GvpC in pET28a
(Plasmid #153294)

• Purpose: Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli
• Depositing Lab: Mikhail Shapiro
• Publication: Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5248
• Total vector size (bp): 5857
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gas vesicle protein C (N-terminal his-tagged)
• Alt name: GvpC
• Species: Anabaena flos-aquae
• Insert Size (bp): 609
• Mutation: Added a hexahistidine tag after the methionine at the N-terminus and has a 1 amino acid glycine linker on either side of the His-tag
• Promoter: T7
• Tag / Fusion Protein:
  • His-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: T7 Terminator Reverse

Resource Information

• Supplemental Documents:
  • WT N-His AnaGvpC in pET28a.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry