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Addgene

pAGM51323
(Plasmid #153224)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153224 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pICH47742
  • Backbone manufacturer
    Marillonnet, Icon Genetics
  • Backbone size w/o insert (bp) 4352
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha ; DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
  • Species
    A. thaliana (mustard weed), Synthetic
  • Insert Size (bp)
    7754

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAGM51323 was a gift from Sylvestre Marillonnet (Addgene plasmid # 153224 ; http://n2t.net/addgene:153224 ; RRID:Addgene_153224)

  • For your References section:

    High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns. Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT, Stuttmann J, Marillonnet S. Plant Commun. 2020 Nov 23;2(2):100135. doi: 10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8. 10.1016/j.xplc.2020.100135 PubMed 33898975
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