pMT-EGFP-Actin5c
(Plasmid #15312)

• Depositing Lab: Ron Vale
• Publication: Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88.

Backbone

• Vector backbone: pMT-V5/His'B'
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3500
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Actin5c
• Alt name: Actin 5c
• Species: D. melanogaster (fly)
• Insert Size (bp): 1871
• Entrez Gene: Act5C (a.k.a. Dmel_CG4027, A, A4V404_DROME, ACT, ACT1_DROME, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, ArpA, BAP47, Bap47, CG4027, Dmel\CG4027, F-ACTIN, F-Actin, F-actin, G-actin, M32055, T11, act, act 5C, act5C, actin, actin5C, actomyosin, anon-EST:fe2D2, beta-Actin, beta-actin, beta-actin/Bap47, chrX:5748184..5748304, cyt5C, f-actin, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486)
• Tags / Fusion Proteins:
  • EGFP (N terminal on insert)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: MT Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry