optGluCl beta Y182F ECFP
(Plasmid #15105)

• Depositing Lab: Henry Lester
• Publication: Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):75-81.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GluCl beta
• Alt name: Glutamate-gated chloride channel alpha
• Alt name: GluCl
• Species: C. elegans (nematode)
• Insert Size (bp): 2200
• Mutation: Codon optimized cDNA, Y182F
• Entrez Gene: glc-2
• Tag / Fusion Protein:
  • ECFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The lab has created an improved version of this plasmid, which can be found at http://www.addgene.org/47542/ .