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pEF myc Gal CDK9 (P#1475)
(Plasmid #14631)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14631 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF-BOS-myc
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cdk9
  • Alt name
    cyclin dependent kinase 9
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDK9 (a.k.a. C-2k, CDC2L4, CTK1, PITALRE, TAK)
  • Tags / Fusion Proteins
    • myc (N terminal on backbone)
    • Gal (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EF1a forward primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gal-Cdk9 expresses Cdk9 fused to the DBD of Gal4 and inserted at the BamHI/XbaI sites of pEF-Bos-myc(EF).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF myc Gal CDK9 (P#1475) was a gift from Matija Peterlin (Addgene plasmid # 14631 ; http://n2t.net/addgene:14631 ; RRID:Addgene_14631)

  • For your References section:

    Interaction between P-TEFb and the C-terminal domain of RNA polymerase II activates transcriptional elongation from sites upstream or downstream of target genes. Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM. Mol Cell Biol. 2002 Jan . 22(1):321-31. 10.1128/MCB.22.1.321-331.2002 PubMed 11739744
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