pC.HA Vav1
(Plasmid #14553)

• Depositing Lab: Joan Brugge
• Publication: Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73.

Backbone

• Vector backbone: pC.HA
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Vav1
• Species: H. sapiens (human)
• Insert Size (bp): 2500
• Entrez Gene: VAV1 (a.k.a. VAV)
• Tag / Fusion Protein:
  • HA (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: SpeI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: human vav1 cDNA was obtained from Amnon Altman
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry