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Addgene

pMX Vav1 Y3F uGFP
(Plasmid #14558)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14558 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMX-uGFP
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vav1
  • Species
    H. sapiens (human)
  • Mutation
    Y3F (Y142, Y160, Y174) in DH domain
  • Entrez Gene
    VAV1 (a.k.a. VAV)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pMX-uGFP was a gift of Eugene Koh
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Gateway cloning system (Invitrogen) was used to subclone human Vav1 into pMX-uGFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX Vav1 Y3F uGFP was a gift from Joan Brugge (Addgene plasmid # 14558 ; http://n2t.net/addgene:14558 ; RRID:Addgene_14558)
  • For your References section:

    An active form of Vav1 induces migration of mammary epithelial cells by stimulating secretion of an epidermal growth factor receptor ligand. Wilsbacher JL, Moores SL, Brugge JS. Cell Commun Signal. 2006 . 4():5. 10.1186/1478-811X-4-5 PubMed 16709244