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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14555 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepC.HA
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVav3
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SpeciesM. musculus (mouse)
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Insert Size (bp)2500
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Entrez GeneVav3 (a.k.a. A530094I06Rik, AA986410, Idd18.1)
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site SpeI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pC.HA Vav3 was a gift from Joan Brugge (Addgene plasmid # 14555 ; http://n2t.net/addgene:14555 ; RRID:Addgene_14555) -
For your References section:
Vav family proteins couple to diverse cell surface receptors. Moores SL, Selfors LM, Fredericks J, Breit T, Fujikawa K, Alt FW, Brugge JS, Swat W. Mol Cell Biol. 2000 Sep . 20(17):6364-73. 10.1128/MCB.20.17.6364-6373.2000 PubMed 10938113