-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14433 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEGFP-N1
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4749
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCaveolin-1
-
Alt nameCav1
-
SpeciesCanis familalis
-
Insert Size (bp)534
-
GenBank IDNM_001003296
-
Entrez GeneCAV1
-
Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site KpnI (destroyed during cloning)
- 5′ sequencing primer 5'-CCAAAATGTCGTAACAACTCCG
- 3′ sequencing primer 5'-CGGACACGCTGAACTTGTGG (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Cav1-GFP was a gift from Ari Helenius (Addgene plasmid # 14433 ; http://n2t.net/addgene:14433 ; RRID:Addgene_14433) -
For your References section:
Assembly and trafficking of caveolar domains in the cell: caveolae as stable, cargo-triggered, vesicular transporters. Tagawa A, Mezzacasa A, Hayer A, Longatti A, Pelkmans L, Helenius A. J Cell Biol. 2005 Aug 29. 170(5):769-79. 10.1083/jcb.200506103 PubMed 16129785
