pPB-C6F/TdTomato
(Plasmid
#140826)
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PurposeOCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 140826 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTRE-Tight-BI
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Backbone manufacturerClontech
- Total vector size (bp) 16425
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameOCT4
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Alt namePOU5F1
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SpeciesH. sapiens (human)
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Insert Size (bp)1080
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GenBank IDNM_002701.6
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Entrez GenePOU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
Gene/Insert 2
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Gene/Insert nameSOX2
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SpeciesH. sapiens (human)
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Insert Size (bp)954
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GenBank IDNM_003106.4
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Entrez GeneSOX2 (a.k.a. ANOP3, MCOPS3)
Gene/Insert 3
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Gene/Insert nameKLF4
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SpeciesH. sapiens (human)
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Insert Size (bp)1410
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GenBank IDNM_004235.6
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Entrez GeneKLF4 (a.k.a. EZF, GKLF)
Gene/Insert 4
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Gene/Insert nameC-MYC
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Alt nameMYC
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SpeciesH. sapiens (human)
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Insert Size (bp)1320
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GenBank IDNM_002467.6
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
Gene/Insert 5
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Gene/Insert nameKLF2
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SpeciesH. sapiens (human)
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Insert Size (bp)1065
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MutationL104P- please see depositor comments
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GenBank IDNM_016270.4
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Entrez GeneKLF2 (a.k.a. LKLF)
Gene/Insert 6
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Gene/Insert nameNANOG
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SpeciesH. sapiens (human)
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Insert Size (bp)918
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Entrez GeneNANOG
Gene/Insert 7
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Gene/Insert nametdTomato
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Insert Size (bp)1431
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor confirms the L104P mutation in KLF2 does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-C6F/TdTomato was a gift from Hideyuki Okano (Addgene plasmid # 140826 ; http://n2t.net/addgene:140826 ; RRID:Addgene_140826) -
For your References section:
Naive-like ESRRB(+) iPSCs with the Capacity for Rapid Neural Differentiation. Kisa F, Shiozawa S, Oda K, Yoshimatsu S, Nakamura M, Koya I, Kawai K, Suzuki S, Okano H. Stem Cell Reports. 2017 Dec 12;9(6):1825-1838. doi: 10.1016/j.stemcr.2017.10.008. Epub 2017 Nov 9. 10.1016/j.stemcr.2017.10.008 PubMed 29129686