pMH0006
(Plasmid #135448)

• Purpose: Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.
• Depositing Labs: Martin Kampmann, Jonathan Weissman
• Publication: Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.009432. doi: 10.1074/jbc.RA119.009432.

Backbone

• Vector backbone: pHR-SFFV-dCas9-BFP-KRAB
• Backbone size w/o insert (bp): 10306
• Total vector size (bp): 15506
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: tagBFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dCas9-BFP-KRAB
• Species: Multiple
• Promoter: EF1A
• Tag / Fusion Protein:
  • tagBFP

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: EF-1a

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry