pGP-FB-orig BA
(Plasmid #13420)

• Depositing Lab: Keith Joung
• Publication: Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259

Backbone

• Vector backbone: pGP-FB
• Backbone size w/o insert (bp): 3483
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: Needs strain with lacIq
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: orig BA
• Alt name: original BCR-ABL three-finger array
• Insert Size (bp): 260
• Tag / Fusion Protein:
  • Gal11P (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GGG GGA TTT CTG TTC ATG GGG G

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Positive control for B2H beta-galactosidase assay. Has coding sequence for �original� BCR-ABL three-finger array.

This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter, which directs expression of the zinc finger-Gal11P hybrid protein.