cytosolic moPrP (3F4)
(Plasmid #1321)

• Depositing Lab: Susan Lindquist
• Publication: Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(26):14955-60. Epub 2001 Dec 11.

Backbone

• Vector backbone: pcb6
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PrP
• Alt name: cytosolic PrP
• Species: M. musculus (mouse)
• Insert Size (bp): 750
• Mutation: N-term truncation (to make gene cytosolic), various mutations to make 3F4 epitope
• GenBank ID: NM_011170
• Entrez Gene: Prnp (a.k.a. CD230, PrP, PrP<C>, PrPC, PrPSc, Prn-i, Prn-p, Sinc, prP27-30, prP33-35C)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: bglII, EcoRI, KpnI, NotI, ClaI (not destroyed)
• 3′ cloning site: HindIII, XbaI, BamHI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The signal sequence at the N-terminus was deleted to make it cytosolic instead of lumenal.