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Addgene

HR180-LGR5-CreER
(Plasmid #129093)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 129093 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HR180-PA
  • Vector type
    homologous recombination donor plasmid
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CreERT2 and LGR5 homology arms
  • Species
    H. sapiens (human)
  • Entrez Gene
    LGR5 (a.k.a. FEX, GPR49, GPR67, GRP49, HG38)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Includes 5' and 3' LGR5 homologous arms.

mRuby is a part of the selection marker mRuby T2A puro, allowing researchers to visually check whether KI is successful.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HR180-LGR5-CreER was a gift from Toshiro Sato (Addgene plasmid # 129093 ; http://n2t.net/addgene:129093 ; RRID:Addgene_129093)

  • For your References section:

    Visualization and targeting of LGR5(+) human colon cancer stem cells. Shimokawa M, Ohta Y, Nishikori S, Matano M, Takano A, Fujii M, Date S, Sugimoto S, Kanai T, Sato T. Nature. 2017 May 11;545(7653):187-192. doi: 10.1038/nature22081. Epub 2017 Mar 29. 10.1038/nature22081 PubMed 28355176
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