pMX1433
(Plasmid #127857)

• Purpose: Expresses EGFP-deltaN-term-MCAK in mammalian cells
• Depositing Lab: Linda Wordeman
• Publication: Domnitz et al J Cell Biol. 2012 Apr 16;197(2):231-7. doi: 10.1083/jcb.201108147. Epub 2012 Apr 9.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 6907
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: deltaN-term-MCAK
• Species: C. griseus
• Insert Size (bp): 2179
• Mutation: deletion of first 19 codons of MCAK
• GenBank ID: U11790.2
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV promoter primer
• 3′ sequencing primer: SV40 polyA reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry