pAM4891 (pCV0003)
(Plasmid #120080)

• Purpose: Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.
• Depositing Lab: Susan Golden
• Publication: Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku673.

Backbone

• Vector backbone: RSF1010Y25F_aph1_PconII-GFPmut2
• Backbone size w/o insert (bp): 7846
• Modifications to backbone: Plasmid assembled from 3 CYANO-VECTOR modular devices: pCVD047 / CV-RSF1010Y25F: The device carries a broad host range replicon derived from RSF1010. A point mutation in mobA prevent self-mobilization. pCVD003 / CV-aph1: The device confers kanamycin or neomycin (Km/Nm) resistance. pCVD030 / CV-PconII-LTRBS-GFPmut2: Promoter/reporter device where GFPmut2 is driven by a PconII promoter. The RBS and flanking sequences are from the pENTR/SD/D-Topo vector (Life Technologies).
• Vector type: Other

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
• Species: Broad range of bacteria
• Mutation: mobA Y25F

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pAM4891 (pcv0003).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry