pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP
(Plasmid
#118594)
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PurposeParental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 118594 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA/Puro-T2A-BFP
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Alt namesgRNA
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Alt namePuro-T2A-BFP
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Insert Size (bp)3012
- Promoter EF1Alpha
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP was a gift from Stanley Qi (Addgene plasmid # 118594 ; http://n2t.net/addgene:118594 ; RRID:Addgene_118594) -
For your References section:
CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming. Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS. Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 10.1016/j.stem.2018.09.003. 10.1016/j.stem.2018.09.003 PubMed 30318302
