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IL13 promoter luciferase
(Plasmid #11784)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11784 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2 Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Bacterial Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IL-13 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    980
  • Entrez Gene
    IL13 (a.k.a. IL-13, P600)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DB Lewis (Dolganov, et al., 1996, PMID 8605348)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human IL-13 5' flank region spanning
-940 to +48.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IL13 promoter luciferase was a gift from Anjana Rao (Addgene plasmid # 11784 ; http://n2t.net/addgene:11784 ; RRID:Addgene_11784)

  • For your References section:

    Gene expression elicited by NFAT in the presence or absence of cooperative recruitment of Fos and Jun. Macian F, Garcia-Rodriguez C, Rao A. EMBO J. 2000 Sep 1. 19(17):4783-95. 10.1093/emboj/19.17.4783 PubMed 10970869
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