pAcGP67A-PSMA
(Plasmid #11524)

• Depositing Lab: Pamela Bjorkman
• Publication: Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 102(17):5981-6.

Backbone

• Vector backbone: pAcGP67A
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 9761
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: prostate-specific membrane antigen
• Alt name: PSMA
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Mutation: There is a short N-terminal truncation of this protein.
• GenBank ID: AAA60209
• Entrez Gene: FOLH1 (a.k.a. FGCP, FOLH, GCP2, GCPII, NAALAD1, PSM, PSMA, mGCP)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: 5' AAT TTAA AAT gAT AAC CAT CTC gC 3''
• 3′ sequencing primer: 5' gAA gCg ggT CCA AgT TTC CCT g 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Joseph Neale and Tomas Bzdega (Georgetown University, Washington, DC)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry