pAcUW31-M10.5/human b2m
(Plasmid #11521)

• Depositing Lab: Pamela Bjorkman
• Publication: Olson et al PLoS Biol. 2005 Aug . 3(8):e257.

Backbone

• Vector backbone: pAcUW31
• Backbone manufacturer: BD Biosciences
• Backbone size w/o insert (bp): 8532
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: M10.5
• Alt name: M10.5 ectodomain
• Alt name: major histocompatibility complex class Ib M10.5
• Species: M. musculus (mouse)
• Insert Size (bp): 948
• GenBank ID: AY211081
• Entrez Gene: H2-M10.4 (a.k.a. 15H)
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: 5' CCg gAT CTg ATC ATg gAg ATA A 3'
• 3′ sequencing primer: 5' TCg CTC TAA CAT ACC ACC CT 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: C. Dulac

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry