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Addgene

pSKB3.TM1777
(Plasmid #11421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSKB3
  • Backbone size w/o insert (bp) 5386
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NusA
  • Species
    T. maritima
  • Insert Size (bp)
    1032
  • GenBank ID
    AAD36840 NP_229574 TM1777
  • Entrez Gene
    nusA
  • Tags / Fusion Proteins
    • 6x His (N terminal on backbone)
    • TEV protease site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the plasmid pSKB3.TM1777 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.

Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSKB3.TM1777 was a gift from Sung-Hou Kim (Addgene plasmid # 11421 ; http://n2t.net/addgene:11421 ; RRID:Addgene_11421)

  • For your References section:

    Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain. Shin DH, Nguyen HH, Jancarik J, Yokota H, Kim R, Kim SH. Biochemistry. 2003 Nov 25. 42(46):13429-37. 10.1021/bi035118h PubMed 14621988
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