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Addgene

GFP::L4440
(Plasmid #11335)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11335 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    L4440
  • Backbone manufacturer
    L4440 is available at Addgene
  • Backbone size w/o insert (bp) 2790
  • Vector type
    Worm Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Alt name
    green fluorescence protein
  • Insert Size (bp)
    869

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Andrew Fire created the vector backbone L4440 and the GFP sequence in vector p95.77
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length GFP sequence (from vector p95.77) was cloned into RNAi feeding vector L4440. Use in a strain carrying a GFP construct to observe reduction of GFP signal after RNAi induction.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP::L4440 was a gift from Guy Caldwell (Addgene plasmid # 11335 ; http://n2t.net/addgene:11335 ; RRID:Addgene_11335)