GFP::L4440
(Plasmid #11335)

• Depositing Lab: Guy Caldwell
• Publication: Integrated Genomics (unpublished)

Backbone

• Vector backbone: L4440
• Backbone manufacturer: L4440 is available at Addgene
• Backbone size w/o insert (bp): 2790
• Vector type: Worm Expression, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Alt name: green fluorescence protein
• Insert Size (bp): 869

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: L4440 primer (AGCGAGTCAGTGAGCGAG)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Andrew Fire created the vector backbone L4440 and the GFP sequence in vector p95.77
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full-length GFP sequence (from vector p95.77) was cloned into RNAi feeding vector L4440. Use in a strain carrying a GFP construct to observe reduction of GFP signal after RNAi induction.

This plasmid is intended for use exclusively as a teaching resource as part of the Integrated Genomics Discovery-Based Laboratory Course. Addgene does not make any guarantee that the plasmid is suitable for research purposes.