pJO427
(Plasmid #111139)

• Purpose: URT-type vector for Glu-mIκBα expression in Mammalian cells
• Depositing Lab: Alexander Varshavsky
• Publication: Piatkov et al Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E817-26. doi: 10.1073/pnas.1401639111. Epub 2014 Feb 18.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: flag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα
• Alt name: Glu51-mIκBα
• Species: M. musculus (mouse)
• Entrez Gene: Ikbkb (a.k.a. IKK-2, IKK-B, IKK-beta, IKK2, IKK[b], IKKbeta, NFKBIKB)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: ClaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry