pJO428
(Plasmid
#111141)
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PurposeURT-type vector for Val-mIκBα expression in Mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111141 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameflag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα
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Alt nameVal51-mIκBα
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SpeciesM. musculus (mouse)
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Entrez GeneIkbkb (a.k.a. IKK-2, IKK-B, IKK-beta, IKK2, IKK[b], IKKbeta, NFKBIKB)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJO428 was a gift from Alexander Varshavsky (Addgene plasmid # 111141 ; http://n2t.net/addgene:111141 ; RRID:Addgene_111141) -
For your References section:
Calpain-generated natural protein fragments as short-lived substrates of the N-end rule pathway. Piatkov KI, Oh JH, Liu Y, Varshavsky A. Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E817-26. doi: 10.1073/pnas.1401639111. Epub 2014 Feb 18. 10.1073/pnas.1401639111 PubMed 24550490