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Addgene

pJO428
(Plasmid #111141)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111141 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    flag-lacZ25-119-DHFR-UbK48R-Val51-mIκBα
  • Alt name
    Val51-mIκBα
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ikbkb (a.k.a. IKK-2, IKK-B, IKK-beta, IKK2, IKK[b], IKKbeta, NFKBIKB)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJO428 was a gift from Alexander Varshavsky (Addgene plasmid # 111141 ; http://n2t.net/addgene:111141 ; RRID:Addgene_111141)
  • For your References section:

    Calpain-generated natural protein fragments as short-lived substrates of the N-end rule pathway. Piatkov KI, Oh JH, Liu Y, Varshavsky A. Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E817-26. doi: 10.1073/pnas.1401639111. Epub 2014 Feb 18. 10.1073/pnas.1401639111 PubMed 24550490