-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 10961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEGFP C1
-
Backbone manufacturerBD Biosciences (Clontech)
- Backbone size w/o insert (bp) 4731
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNFAT3
-
Alt namenuclear factor of activated T cells 3
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2700
-
GenBank IDL41066.1
-
Entrez GeneNFATC4 (a.k.a. NF-AT3, NF-ATC4, NFAT3)
-
Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byNFAT3 was a gift from Jeffery D. Molkentin
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEGFP-C1 NFAT3 was a gift from Toren Finkel (Addgene plasmid # 10961 ; http://n2t.net/addgene:10961 ; RRID:Addgene_10961) -
For your References section:
Ras regulates NFAT3 activity in cardiac myocytes. Ichida M, Finkel T. J Biol Chem. 2001 Feb 2. 276(5):3524-30. 10.1074/jbc.M004275200 PubMed 11044444