NFAT luciferase reporter
(Plasmid #10959)

• Depositing Lab: Toren Finkel
• Publication: Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Backbone

• Vector backbone: pGL2-Basic
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IL-2 promoter
• Alt name: IL-2
• Species: H. sapiens (human)
• Insert Size (bp): 90
• Entrez Gene: IL2 (a.k.a. IL-2, TCGF, lymphokine)
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LucNRev

Resource Information

• Articles Citing this Plasmid:
  • 22 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A luciferase reporter containing three tandem repeats of a 30-bp fragment of the IL-2 promoter for analysis of NFAT activity. 30-bp fragment is GGAGG AAAAA CTGTT TCATA CAGAA GGCGT.