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Addgene

NFAT luciferase reporter
(Plasmid #10959)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10959 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL2-Basic
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IL-2 promoter
  • Alt name
    IL-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    90
  • Entrez Gene
    IL2 (a.k.a. IL-2, TCGF, lymphokine)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A luciferase reporter containing three tandem repeats of a 30-bp fragment of the IL-2 promoter for analysis of NFAT activity. 30-bp fragment is GGAGG AAAAA CTGTT TCATA CAGAA GGCGT.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NFAT luciferase reporter was a gift from Toren Finkel (Addgene plasmid # 10959 ; http://n2t.net/addgene:10959 ; RRID:Addgene_10959)
  • For your References section:

    Ras regulates NFAT3 activity in cardiac myocytes. Ichida M, Finkel T. J Biol Chem. 2001 Feb 2. 276(5):3524-30. 10.1074/jbc.M004275200 PubMed 11044444