pDEST47-CYFIP1.GFP
(Plasmid #109139)

• Purpose: GFP-tagged CYFIP1 expression construct
• Depositing Lab: Josef Kittler
• Publication: Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. doi: 10.1038/tp.2014.16.

Backbone

• Vector backbone: pDEST47
• Backbone manufacturer: Invitrogen
• Total vector size (bp): 9894
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CYFIP1
• Alt name: cytoplasmic FMR1 interacting protein 1
• Alt name: SRA1
• Species: H. sapiens (human)
• Insert Size (bp): 4519
• GenBank ID:
• Entrez Gene: CYFIP1 (a.k.a. P140SRA-1, SHYC, SRA-1, SRA1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: GFP-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry