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Addgene

pDisplay-R-iGluSnFR1
(Plasmid #107335)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 107335 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDisplay
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5268
  • Total vector size (bp) 6819
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    R-iGluSnFR1
  • Alt name
    cpmApple based glutamate sensor
  • Species
    Synthetic
  • Insert Size (bp)
    1641
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutation M178K in the Neomycin resistance cassette was found during Addgene's quality control. Neomycin selection has not been tested for this plasmid, but should not affect sensor function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisplay-R-iGluSnFR1 was a gift from Robert Campbell (Addgene plasmid # 107335 ; http://n2t.net/addgene:107335 ; RRID:Addgene_107335)

  • For your References section:

    Genetically Encoded Glutamate Indicators with Altered Color and Topology. Wu J, Abdelfattah AS, Zhou H, Ruangkittisakul A, Qian Y, Ballanyi K, Campbell RE. ACS Chem Biol. 2018 Jan 22. doi: 10.1021/acschembio.7b01085. 10.1021/acschembio.7b01085 PubMed 29308878
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