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hygII-UT-ABAleon2.15
(Plasmid #106983)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    hygII-UT
  • Total vector size (bp) 15280
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ABAleon2.1
  • Species
    Synthetic
  • Insert Size (bp)
    3057
  • Promoter UBQ10

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Spe I/Xba I (destroyed during cloning)
  • 3′ cloning site Sac I (not destroyed)
  • 5′ sequencing primer gctttctctttgcgcttgcg
  • 3′ sequencing primer ggagtcacgttatgac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hygII-UT-ABAleon2.15 was a gift from Julian Schroeder (Addgene plasmid # 106983 ; http://n2t.net/addgene:106983 ; RRID:Addgene_106983)

  • For your References section:

    FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis. Waadt R, Hitomi K, Nishimura N, Hitomi C, Adams SR, Getzoff ED, Schroeder JI. Elife. 2014 Apr 15;3:e01739. doi: 10.7554/eLife.01739. 10.7554/eLife.01739 PubMed 24737861
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