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Addgene

pEGFP-C1-ER beta
(Plasmid #28237)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28237 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5 alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ER beta
  • Alt name
    ESR2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1590
  • GenBank ID
    NM_001437
  • Entrez Gene
    ESR2 (a.k.a. ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho I (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pSG5-ER beta, Jan-Ake Gustafsson
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-ER beta was a gift from Michael Mancini (Addgene plasmid # 28237 ; http://n2t.net/addgene:28237 ; RRID:Addgene_28237)