pEMS2144
(Plasmid #104357)

• Purpose: AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter
• Depositing Lab: Elizabeth Simpson
• Publication: de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4.

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Backbone

• Vector backbone: AAV2
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): SURE
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ssAAV-smCBA-EmGFP
• Alt name: EmGFP
• Species: Synthetic
• Promoter: smCBA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: EBV-rev

Resource Information

• Supplemental Documents:
  • pEMS2144.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid.