mito-mGarnet2
(Plasmid
#104310)
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Purposecodes for a fusion of fluorescent protein mGarnet2 and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104310 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen/ life technologies
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemito-mGarnet2
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Insert Size (bp)807
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG
- 3′ sequencing primer GGC AAC TAG AAG GCA CAG TC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mito-mGarnet2 was a gift from Ulrich Nienhaus (Addgene plasmid # 104310 ; http://n2t.net/addgene:104310 ; RRID:Addgene_104310) -
For your References section:
A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy. Matela G, Gao P, Guigas G, Eckert AF, Nienhaus K, Nienhaus GU. Chem Commun (Camb). 2017 Jan 10;53(5):979-982. doi: 10.1039/c6cc09081h. 10.1039/c6cc09081h PubMed 28044150
