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Addgene

PPRE X3-TK-luc
(Plasmid #1015)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1015 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ptkLUC (modified?)
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PPRE
  • Alt name
    PPRE-luc
  • Alt name
    DR1X3
  • Alt name
    PPAR response element
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    100

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

3xDR-1 response elements (AGGACAAAGGTCA) upstream of a luciferase reporter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PPRE X3-TK-luc was a gift from Bruce Spiegelman (Addgene plasmid # 1015 ; http://n2t.net/addgene:1015 ; RRID:Addgene_1015)

  • For your References section:

    ADD1/SREBP1 activates PPARgamma through the production of endogenous ligand. Kim JB, Wright HM, Wright M, Spiegelman BM. Proc Natl Acad Sci U S A 1998 Apr 14;95(8):4333-7. 10.1073/pnas.95.8.4333 PubMed 9539737
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