pDONOR-tagBFP-PSM-EGFP
(Plasmid
#100603)
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Purpose(Empty Backbone) Donor scaffold with EGFP positive selection module, and tagBFP negative selection module
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100603 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC-AMPr based
- Backbone size (bp) 2898
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Modifications to backboneInsert NSM (negative selection module) and HpaI flanked PSM (ITR positive selection module ITR)
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Vector typeSynthetic Biology
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Selectable markersPuromycin
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Tags
/ Fusion Proteins
- tagBFP
- EGFP-T2A-Puro
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONOR-tagBFP-PSM-EGFP was a gift from Jens Schwamborn (Addgene plasmid # 100603 ; http://n2t.net/addgene:100603 ; RRID:Addgene_100603) -
For your References section:
FACS-Assisted CRISPR-Cas9 Genome Editing Facilitates Parkinson's Disease Modeling. Arias-Fuenzalida J, Jarazo J, Qing X, Walter J, Gomez-Giro G, Nickels SL, Zaehres H, Scholer HR, Schwamborn JC. Stem Cell Reports. 2017 Nov 14;9(5):1423-1431. doi: 10.1016/j.stemcr.2017.08.026. Epub 2017 Oct 5. 10.1016/j.stemcr.2017.08.026 PubMed 28988985
Map uploaded by the depositor.
