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Addgene

FL MALT1 WT in pET29b-Strep
(Plasmid #48968)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 48968 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET29b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5371
  • Total vector size (bp) 7903
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MALT1 isoform A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2532
  • Entrez Gene
    MALT1 (a.k.a. IMD12, MLT, MLT1, PCASP1)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (C terminal on backbone)
    • Strep (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer T7 Reverse
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FL MALT1 WT in pET29b-Strep was a gift from Guy Salvesen (Addgene plasmid # 48968 ; http://n2t.net/addgene:48968 ; RRID:Addgene_48968)
  • For your References section:

    Mechanism and specificity of the human paracaspase MALT1. Hachmann J, Snipas SJ, van Raam BJ, Cancino EM, Houlihan EJ, Poreba M, Kasperkiewicz P, Drag M, Salvesen GS. Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. 10.1042/BJ20120035 PubMed 22309193