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Addgene

Flag-MALT1-/pRK5
(Plasmid #27971)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27971 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MALT1
  • Species
    H. sapiens (human)
  • Entrez Gene
    MALT1 (a.k.a. IMD12, MLT, MLT1, PCASP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-MALT1-/pRK5 was a gift from Xiaolu Yang (Addgene plasmid # 27971 ; http://n2t.net/addgene:27971 ; RRID:Addgene_27971)
  • For your References section:

    The paracaspase MALT1 controls caspase-8 activation during lymphocyte proliferation. Kawadler H, Gantz MA, Riley JL, Yang X. Mol Cell. 2008 Aug 8. 31(3):415-21. 10.1016/j.molcel.2008.06.008 PubMed 18691973