pET21 Catalytic Domain of MALT1 Catalytic Mutant
(Plasmid
#48971)
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PurposeExpresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48971 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5443
- Total vector size (bp) 6199
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMALT1
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SpeciesH. sapiens (human)
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Insert Size (bp)756
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MutationContains AA 329-566; Catalytic Mutant C464A
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Entrez GeneMALT1 (a.k.a. IMD12, MLT, MLT1, PCASP1)
- Promoter T7
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 Forward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21 Catalytic Domain of MALT1 Catalytic Mutant was a gift from Guy Salvesen (Addgene plasmid # 48971 ; http://n2t.net/addgene:48971 ; RRID:Addgene_48971) -
For your References section:
Mechanism and specificity of the human paracaspase MALT1. Hachmann J, Snipas SJ, van Raam BJ, Cancino EM, Houlihan EJ, Poreba M, Kasperkiewicz P, Drag M, Salvesen GS. Biochem J. 2012 Apr 1;443(1):287-95. doi: 10.1042/BJ20120035. 10.1042/BJ20120035 PubMed 22309193