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PurposeNegative feedback plasmid from oscillator
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48884 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZ vector
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameaiiA and LuxR
- Promoter pLux
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Tag
/ Fusion Protein
- ssrA (TSAANDENYALAA) (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer no info
- 3′ sequencing primer no info (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTD103aiiA was a gift from Jeff Hasty (Addgene plasmid # 48884 ; http://n2t.net/addgene:48884 ; RRID:Addgene_48884) -
For your References section:
A synchronized quorum of genetic clocks. Danino T, Mondragon-Palomino O, Tsimring L, Hasty J. Nature. 2010 Jan 21;463(7279):326-30. doi: 10.1038/nature08753. 10.1038/nature08753 PubMed 20090747