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PurposePositive feedback plasmid from oscillator
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZ vector
- Total vector size (bp) 4885
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namearaC gene with ssrA tag
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Insert Size (bp)918
- Promoter Para/lac
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Tag
/ Fusion Protein
- ssrA (TSAANDENYALAA) (C terminal on insert)
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer no info
- 3′ sequencing primer no info (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namemonomeric yeast-enhanced green fluorescent protein
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Tag
/ Fusion Protein
- ssrA (TSAANDENYALAA) (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJS167 was a gift from Jeff Hasty (Addgene plasmid # 48881 ; http://n2t.net/addgene:48881 ; RRID:Addgene_48881) -
For your References section:
A fast, robust and tunable synthetic gene oscillator. Stricker J, Cookson S, Bennett MR, Mather WH, Tsimring LS, Hasty J. Nature. 2008 Nov 27;456(7221):516-9. doi: 10.1038/nature07389. Epub 2008 Oct 29. 10.1038/nature07389 PubMed 18971928