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PurposePositive feedback plasmid from oscillator
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 48885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZ vector
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsLB should be supplemented with 0.2% glucose *
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLuxI, sfGFP, and LuxR
- Promoter pLux
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Tag
/ Fusion Protein
- ssrA tag (AANDENYALAA) (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer no info
- 3′ sequencing primer no info (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
* The presence of glucose will reduce the amount of expression from the Lux promoter driving GFP and other genes. Omitting the glucose could potentially make the cell overburdened.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTD103luxI_sfGFP was a gift from Jeff Hasty (Addgene plasmid # 48885 ; http://n2t.net/addgene:48885 ; RRID:Addgene_48885) -
For your References section:
A sensing array of radically coupled genetic 'biopixels'. Prindle A, Samayoa P, Razinkov I, Danino T, Tsimring LS, Hasty J. Nature. 2011 Dec 18;481(7379):39-44. doi: 10.1038/nature10722. 10.1038/nature10722 PubMed 22178928