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Addgene

pDEINO4
(Plasmid #179488)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179488 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDEINO1
  • Backbone manufacturer
    Brumwell et al.
  • Backbone size w/o insert (bp) 14930
  • Total vector size (bp) 16996
  • Modifications to backbone
    Addition of spectinomycin/streptomycin resistance gene. Removal of algae selective marker (Nourseothricin), S. meliloti origin of replication (RepA2B2C2) and NptII selective marker.
  • Vector type
    Synthetic Biology ; Bacterial and Yeast Cloning
  • Selectable markers
    HIS3 ; Chloramphenicol - D. radiodurans, E. coli; Streptomycin - D. radiodurans

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Epi300
  • Growth instructions
    Plasmid can be induced to high copy with arabinose.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    aadA1
  • Alt name
    Spectinomycin/Streptomycin resistance gene
  • Alt name
    Aminoglycoside adenylyltransferase
  • Insert Size (bp)
    2066

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Frey and Krisch Lab (SpecR/StrepR resistance gene was cloned from the Ω cassette). Reference: Frey, J., & Krisch, H. M. (1985). Ω mutagenesis in gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene, 36(1-2), 143-150.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEINO4 was a gift from Bogumil Karas (Addgene plasmid # 179488 ; http://n2t.net/addgene:179488 ; RRID:Addgene_179488)
  • For your References section:

    Conjugation-Based Genome Engineering in Deinococcus radiodurans. Brumwell SL, Van Belois KD, Giguere DJ, Edgell DR, Karas BJ. ACS Synth Biol. 2022 Mar 18;11(3):1068-1076. doi: 10.1021/acssynbio.1c00524. Epub 2022 Mar 7. 10.1021/acssynbio.1c00524 PubMed 35254818