pDEINO4
(Plasmid
#179488)
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PurposeMulti-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179488 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEINO1
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Backbone manufacturerBrumwell et al.
- Backbone size w/o insert (bp) 14930
- Total vector size (bp) 16996
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Modifications to backboneAddition of spectinomycin/streptomycin resistance gene. Removal of algae selective marker (Nourseothricin), S. meliloti origin of replication (RepA2B2C2) and NptII selective marker.
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Vector typeSynthetic Biology ; Bacterial and Yeast Cloning
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Selectable markersHIS3 ; Chloramphenicol - D. radiodurans, E. coli; Streptomycin - D. radiodurans
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Epi300
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Growth instructionsPlasmid can be induced to high copy with arabinose.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameaadA1
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Alt nameSpectinomycin/Streptomycin resistance gene
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Alt nameAminoglycoside adenylyltransferase
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Insert Size (bp)2066
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byFrey and Krisch Lab (SpecR/StrepR resistance gene was cloned from the Ω cassette). Reference: Frey, J., & Krisch, H. M. (1985). Ω mutagenesis in gram-negative bacteria: a selectable interposon which is strongly polar in a wide range of bacterial species. Gene, 36(1-2), 143-150.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEINO4 was a gift from Bogumil Karas (Addgene plasmid # 179488 ; http://n2t.net/addgene:179488 ; RRID:Addgene_179488) -
For your References section:
Conjugation-Based Genome Engineering in Deinococcus radiodurans. Brumwell SL, Van Belois KD, Giguere DJ, Edgell DR, Karas BJ. ACS Synth Biol. 2022 Mar 18;11(3):1068-1076. doi: 10.1021/acssynbio.1c00524. Epub 2022 Mar 7. 10.1021/acssynbio.1c00524 PubMed 35254818