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Addgene

pDEINO1
(Plasmid #179472)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179472 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAGE3.0
  • Backbone manufacturer
    Brumwell et al.
  • Backbone size w/o insert (bp) 17515
  • Total vector size (bp) 22148
  • Modifications to backbone
    Addition of D. radiodurans origin of replication and codon-optimized chloramphenicol resistance gene.
  • Vector type
    Synthetic Biology ; Bacterial and Yeast Cloning
  • Selectable markers
    HIS3 ; Chloramphenicol - D. radiodurans, E. coli; Neomycin/Kan - D. radiodurans, E. coli, S. meliloti; Nourseothricin (NAT) - algae

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Epi300
  • Growth instructions
    Plasmid can be induced to high copy with arabinose.
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    ori
  • Alt name
    D. radiodurans origin of replication
  • Species
    D. radiodurans
  • Insert Size (bp)
    3810

Gene/Insert 2

  • Gene/Insert name
    cat
  • Alt name
    Chloramphenicol resistance gene codon-optimized for D. radiodurans
  • Species
    Synthetic
  • Insert Size (bp)
    823

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Mary Lidstrom's Lab at the University of Washington (D. radiodurans origin of replication was cloned from pRAD1) and Murray Junop's Lab at Western University (D. radiodurans codon-optimized chloramphenicol gene was cloned from pRadDEST-GFP). Reference: Meima, R., & Lidstrom, M. E. (2000). Characterization of the minimal replicon of a cryptic Deinococcus radiodurans SARK plasmid and development of versatile Escherichia coli-D. radiodurans shuttle vectors. Applied and environmental microbiology, 66(9), 3856-3867.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEINO1 was a gift from Bogumil Karas (Addgene plasmid # 179472 ; http://n2t.net/addgene:179472 ; RRID:Addgene_179472)
  • For your References section:

    Conjugation-Based Genome Engineering in Deinococcus radiodurans. Brumwell SL, Van Belois KD, Giguere DJ, Edgell DR, Karas BJ. ACS Synth Biol. 2022 Mar 18;11(3):1068-1076. doi: 10.1021/acssynbio.1c00524. Epub 2022 Mar 7. 10.1021/acssynbio.1c00524 PubMed 35254818