pDEINO1
(Plasmid
#179472)
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PurposeMulti-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAGE3.0
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Backbone manufacturerBrumwell et al.
- Backbone size w/o insert (bp) 17515
- Total vector size (bp) 22148
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Modifications to backboneAddition of D. radiodurans origin of replication and codon-optimized chloramphenicol resistance gene.
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Vector typeSynthetic Biology ; Bacterial and Yeast Cloning
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Selectable markersHIS3 ; Chloramphenicol - D. radiodurans, E. coli; Neomycin/Kan - D. radiodurans, E. coli, S. meliloti; Nourseothricin (NAT) - algae
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Epi300
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Growth instructionsPlasmid can be induced to high copy with arabinose.
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameori
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Alt nameD. radiodurans origin of replication
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SpeciesD. radiodurans
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Insert Size (bp)3810
Gene/Insert 2
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Gene/Insert namecat
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Alt nameChloramphenicol resistance gene codon-optimized for D. radiodurans
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SpeciesSynthetic
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Insert Size (bp)823
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byMary Lidstrom's Lab at the University of Washington (D. radiodurans origin of replication was cloned from pRAD1) and Murray Junop's Lab at Western University (D. radiodurans codon-optimized chloramphenicol gene was cloned from pRadDEST-GFP). Reference: Meima, R., & Lidstrom, M. E. (2000). Characterization of the minimal replicon of a cryptic Deinococcus radiodurans SARK plasmid and development of versatile Escherichia coli-D. radiodurans shuttle vectors. Applied and environmental microbiology, 66(9), 3856-3867.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEINO1 was a gift from Bogumil Karas (Addgene plasmid # 179472 ; http://n2t.net/addgene:179472 ; RRID:Addgene_179472) -
For your References section:
Conjugation-Based Genome Engineering in Deinococcus radiodurans. Brumwell SL, Van Belois KD, Giguere DJ, Edgell DR, Karas BJ. ACS Synth Biol. 2022 Mar 18;11(3):1068-1076. doi: 10.1021/acssynbio.1c00524. Epub 2022 Mar 7. 10.1021/acssynbio.1c00524 PubMed 35254818