pDEINO3
(Plasmid
#179487)
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PurposeMulti-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179487 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDEINO1
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Backbone manufacturerBrumwell et al.
- Backbone size w/o insert (bp) 14930
- Total vector size (bp) 17215
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Modifications to backboneAddition of tetracycline resistance gene. Removal of algae selective marker (Nourseothricin), S. meliloti origin of replication (RepA2B2C2) and NptII selective marker.
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Vector typeSynthetic Biology ; Bacterial and Yeast Cloning
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Selectable markersHIS3 ; Chloramphenicol - D. radiodurans, E. coli; Tetracycline - D. radiodurans, E. coli
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Epi300
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Growth instructionsPlasmid can be induced to high copy with arabinose.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTetR/A
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Alt nameTetracycline resistance gene
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SpeciesPseudomonas aeruginosa
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Insert Size (bp)2285
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byJones and Gutterson (Tetracycline resistance gene was cloned from pRK7813). Reference: Jones, J. D., & Gutterson, N. (1987). An efficient mobilizable cosmid vector, pRK7813, and its use in a rapid method for marker exchange in Pseudomonas fluorescens strain HV37a. Gene, 61(3), 299-306.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEINO3 was a gift from Bogumil Karas (Addgene plasmid # 179487 ; http://n2t.net/addgene:179487 ; RRID:Addgene_179487) -
For your References section:
Conjugation-Based Genome Engineering in Deinococcus radiodurans. Brumwell SL, Van Belois KD, Giguere DJ, Edgell DR, Karas BJ. ACS Synth Biol. 2022 Mar 18;11(3):1068-1076. doi: 10.1021/acssynbio.1c00524. Epub 2022 Mar 7. 10.1021/acssynbio.1c00524 PubMed 35254818