Skip to main content
Addgene

pDEINO3
(Plasmid #179487)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179487 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDEINO1
  • Backbone manufacturer
    Brumwell et al.
  • Backbone size w/o insert (bp) 14930
  • Total vector size (bp) 17215
  • Modifications to backbone
    Addition of tetracycline resistance gene. Removal of algae selective marker (Nourseothricin), S. meliloti origin of replication (RepA2B2C2) and NptII selective marker.
  • Vector type
    Synthetic Biology ; Bacterial and Yeast Cloning
  • Selectable markers
    HIS3 ; Chloramphenicol - D. radiodurans, E. coli; Tetracycline - D. radiodurans, E. coli

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Epi300
  • Growth instructions
    Plasmid can be induced to high copy with arabinose.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TetR/A
  • Alt name
    Tetracycline resistance gene
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    2285

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Jones and Gutterson (Tetracycline resistance gene was cloned from pRK7813). Reference: Jones, J. D., & Gutterson, N. (1987). An efficient mobilizable cosmid vector, pRK7813, and its use in a rapid method for marker exchange in Pseudomonas fluorescens strain HV37a. Gene, 61(3), 299-306.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEINO3 was a gift from Bogumil Karas (Addgene plasmid # 179487 ; http://n2t.net/addgene:179487 ; RRID:Addgene_179487)
  • For your References section:

    Conjugation-Based Genome Engineering in Deinococcus radiodurans. Brumwell SL, Van Belois KD, Giguere DJ, Edgell DR, Karas BJ. ACS Synth Biol. 2022 Mar 18;11(3):1068-1076. doi: 10.1021/acssynbio.1c00524. Epub 2022 Mar 7. 10.1021/acssynbio.1c00524 PubMed 35254818