...the chamber of the SDS-PAGE gel tank. Pro-Tip Different SDS-PAGE gel tanks will have different gel orientations...and pipettes Spectrophotometer Heat block Mini gel tank chamber Power supply iBlot 2 Gel Transfer Device...orientations. Refer to the instructions for your specific tank. Close the clamp. Prepare 1X running buffer as follows.... Mix well. Fill the chamber of the SDS-PAGE gel tank with the 1X running buffer. Load the samples into... prestained protein ladder. Place the lid on the tank and plug the electrode cords into the power supply...